Fluorescence microfluorometry combines the spatial resolution and light collection of a microscope with the analytical power of spectrofluorometry. Unlike conventional fluorescence microscopy which gives images at one or several wavelength bands, Horiba Scientific's microfluorometry solutions can give you complete steady state spectra or lifetime from any point in the image.
We have over 40 years of experience in sales and technical support of scientific instrumentation . We can help you decide on the appropriate equipment for your needs.
Time-resolved fluorescence microscopy is the ultimate tool to study dynamic events in cellular structures and nanomaterials. Unlike conventional intensity based fluorescence microscopy, fluorescence lifetime is an intrinsic property of a fluorophore, and therefore its measurement is not affected by non-uniform loading, photobleaching, excitation light instability, and light scattering. More importantly, time-resolved measurements can provide essential dynamic information about a fluorophore’s microenvironment and its interaction with other molecules. HORIBA Scientific, the leader in fluorescence spectroscopy, offers the DeltaMyc™, a filter-based confocal microscope system to measure fluorescence lifetime and intensity in micro scales.
The DeltaMyc features time-correlated single photon counting (TCSPC) for sensitive and rapid acquisition of luminescence lifetimes from 100 ps to seconds. It is composed of a compact optical module added to an Olympus BX 53 upright microscope or IX73 inverted microscope. Its mapping capabilities include an automated X,Y fast scanning stage, which combined with its confocal ability, can generate fluorescence lifetime mapping with micron level spatial resolution.
The DeltaMyc is a flexible research tool that combines a large range of picosecond pulsed laser diode sources spanning wavelengths from 370 to 980 nm and repetition rates from CW to 100 MHz, multiple filter configurations and various detector options to suit your specific measurement needs. An included high dynamic range, low noise, cooled camera and a high intensity fluorescence illuminator configured with the DeltaMyc enable widefield fluorescence imaging.
The DeltaMyc features fully-automated mapping controlled from the intuitive user interface of our DataStation software, while retaining the full flexibility and functionality of the microscope. Full reconvolution analysis can be performed to generate maps of the fit parameters such as lifetimes, relative amplitudes, average lifetime, and fluorescence intensity. For example, it is ideal an instrument study protein dynamics such as binding or disassociation using Fluorescence Resonance Energy Transfer (FRET).
Calcium Ratio Imaging Microscopy System
The EasyRatioPro is the most comprehensive system for measuring intracellular calcium, sodium, magnesium ion concentrations or pH and membrane potential. EasyRatioPro includes all the tools required for image acquisition, analysis and calibration of intracellular dyes or intrinsic fluorophores
The EasyRatioPro is a monochromator-based wide-field microscope system for measuring ratio-fluorescence or fluorescence dye intensity of labeled proteins in nanomolar concentrations. Ratio-fluorescence microscopy is the ultimate tool to study dynamic events in live cells and cellular structures. Unlike conventional intensity based fluorescence microscopy, ratio-fluorescence eliminates the effects of photo-bleaching, cell path length variation, non-uniformity of dye loading, non-uniform field of view, making ratio-fluorescence the most accurate method to determine intracellular ion concentrations. More importantly, EasyRatioPro is fast enough to provide essential dynamic information about ion concentrations and interactions with other molecules, drugs or added reagents.
The EasyRatioPro incorporates CCD, sCMOS or EMCCD cameras for sensitive and rapid acquisition of fluorescence images from up to 16 different excitation wavelengths and optionally, four emission wavelengths. (Over 100 cameras are supported, with more added routinely.) Excitation is via our patented DeltaRam™ X compact rapid switching monochromator coupled by a liquid light guide to an upright or inverted fluorescence microscope. This allows virtually infinite excitation wavelengths from 250 to 650 nm, and acquisition rates of up to 250 for dual excitation measurements or 1000 images per second for dual emission.
Another exclusive innovation of EasyRatioPro is our patented “Warp Drive” that revolutionizes imaging by increasing productivity through its innovative tactile control surface. Warp drive features real-time control of up to 60 programmable function keys and touch sensitive sliders to control exposure time, binning, gain, and wavelength selection of up to 16 different channels. It’s never been easier to optimize your images in real time!
PTI RatioMaster is a monochromator-based wide-field microscope system for measuring ratio-fluorescence or fluorescence dye intensity of labeled proteins in nanomolar concentrations. Ratio-fluorescence microscopy is the ultimate tool to study dynamic events in live cells and cellular structures. Unlike conventional intensity based fluorescence microscopy, ratio-fluorescence eliminates the effects of photo-bleaching, cell path length variation, non-uniformity of dye loading, non-uniform field of view, making ratio-fluorescence the most accurate method to determine intracellular ion concentrations. More importantly, PTI RatioMaster is fast enough to provide essential dynamic information about ion concentrations and interactions with other molecules, drugs or added reagents.
The PTI RatioMaster is capable of dynamic ratio fluorescence measurements on a millisecond timescale. A xenon arc lamp provides high intensity, continuous broadband illumination. Alternating excitation wavelengths are selected by a computer-controlled high-speed random access monochromator coupled to a (user specified/supplied) inverted or upright, fluorescence microscope with a liquid light guide. Emitted light is collected from the sample and passed through a photometer having a bilateral translatable iris and a viewing eyepiece to a switchable analog/photon counting photomultiplier detector. Analog detection is used when emitted light is relatively intense; low light levels are detected by photon counting. All system functions are under computer control. Data is collected and analyzed by proprietary Windows “FelixGX” based advanced fluorescence software.
An optional Sarcomere Length module is available to measure the changes in sarcomere length and whole cell length as a function of time. This allows correlation of contractility information with calcium concentration.
We can work with you to help get the ideal instruments into your university by providing any information you require.
Quark Photonics is a leading supplier of scientific instrumentation serving the Australian and New Zealand scientific research, defence, industrial and life science markets, specialising in spectroscopy and photonics instrumentation.
With an innovative and industry leading network of suppliers and our experienced sales and service team we have a comprehensive product portfolio to address even the most demanding of scientific applications.